Thymidine kinase mutants obtained by random sequence selection.
نویسندگان
چکیده
منابع مشابه
Herpes simplex virus-1 thymidine kinase mutants created by semi-random sequence mutagenesis improve prodrug-mediated tumor cell killing.
Cancer suicide gene therapy affords the prospect of using the most optimal genes available because the source of the therapeutic gene is often irrelevant. Currently, there are numerous preclinical and clinical trials to develop tumor ablative therapies that use viral, yeast, or bacterial genes. One such gene, the herpes simplex virus type 1 (HSV-1) thymidine kinase (TK) is widely used as a suic...
متن کاملProdrug-mediated Tumor Cell Killing Semi-Random Sequence Mutagenesis Improve Herpes Simplex Virus-1 Thymidine Kinase Mutants Created by Updated Version
Cancer suicide gene therapy affords the prospect of using the most optimal genes available because the source of the therapeutic gene is often irrelevant. Currently, there are numerous preclinical and clinical trials to develop tumor ablative therapies that use viral, yeast, or bacterial genes. One such gene, the herpes simplex virus type 1 (HSV-1) thymidine kinase (TK) is widely used as a suic...
متن کاملMouse thymidine kinase: the promoter sequence and the gene and pseudogene structures in normal cells and in thymidine kinase deficient mutants.
The mouse genome carries one gene and two pseudogenes for cytoplasmic thymidine kinase. The overall structure of these genes was determined with the help of cosmids and lambda phage clones and the upstream sequence containing the promoter was determined. The data allow an allocation of bands seen in the complex patterns of genomic Southern blots obtained from the DNA of wild type cells and of t...
متن کاملFeedback Inhibition of Thymidine Kinase by Thymidine Triphosphate.
That the regulation of cell division and deoxyribonucleic acid synthesis is subject to some kind of chemical control in higher organisms is widely accepted (2-5). Davidson (2) has suggested that deoxyribonucleic acid synthesis takes place at critical concentrations of the deoxyribonucleoside triphosphates and that these compounds are components of a homeostatic mechanism. There are now at least...
متن کاملFeedback Inhibition of Thymidine Kinase by Thymidine T&phosphate*
That the regulation of cell division and deoxyribonucleic acid synthesis is subject to some kind of chemical control in higher organisms is widely accepted (2-5). Davidson (2) has suggested that deoxyribonucleic acid synthesis takes place at critical concentrations of the deoxyribonucleoside triphosphates and that these compounds are components of a homeostatic mechanism. There are now at least...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1993
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.90.9.4012